The experimental study of the specific pharmacological action of «Artproment» combined gel

Authors

  • О. S. Shpychak
  • O. I. Tikhonov

DOI:

https://doi.org/10.14739/2310-1210.2014.4.27460

Keywords:

Sports Injuries, Analgesic, Local Anesthetic, Decongestants, Sports Medicine

Abstract

The preclinical experimental research concerning the study of the specific pharmacological action of "ArtpromentÒ" gel has been done. The gel developed contains propolis phenolic hydrophobic drug, articaine hydrochloride, menthol and rosemary oil in its composition. The medicine is planned to application in sports medicine with the purpose of treating traumatic injuries of organs of the locomotor apparatus accompanied with sport traumas (bruises, dislocations, sprains, ruptures of ligaments, tendons, etc.).

In this work the pharmacological, physiological, instrumental methods of research and methods of mathematical statistics have been used.

The study of the analgesic and local anesthetic action of the gel developed was conducted at the premises of the Central Research Laboratory of NUPh under the supervision of Doctor of Pharmacy, professor L.V.Iakovlieva on the model of tail thermoirritation in white rats weighing 180.0-200.0 g. The analgesic and local anesthetic actions were estimated by the ability of the medicine studied to change the pain threshold (duration of the latent period, sеc.) in animals of the test group comparing to the control group in the dose of 25 mg/cm2 and the base in the dose of 30 mg/cm2 after application to the tail.

The results of the experiment testify that when studying the influence of the combined gel on the course of the experimental nociceptive response of the central origin the significant analgesic action of "ArtpromentÒ" medicine has been determined; its highest intensity was observed in 2.5 and 3.5 hours after application and is 52 % and 59 %, respectively. Despite the fact that the progress of the pain syndrome in the model of tail thermoirritation is mediated by the central nervous system, it is possible to conclude that the developed gel has an expressed analgesic and local anesthetic action. The medicine shows the significant anti-edema action at the level of the reference medicine "Diclofen-gel" on the carrageenan-induced paw oedema model.

Taking into account that injuries of organs of the locomotor apparatus cause manifestation of inflammation and development of oedema it is expedient to study the anti-edema action of "ArtpromentÒ" gel in comparison with "Diclofen-gel", which is the complete analogue by the specific pharmacological action and the partial analogue by the composition. The research was conducted using the carrageenan-induced paw edema model in 24 rats weighing 180.0-200.0 g. Edema was caused by subplantar introduction of 0.1 ml of 1 % solution of carrageenin into one of the hind limbs of the animals of the test and control groups. Development of oedema was registered by increase of the paw’s volume measured in dynamics in 1; 2; 3; 4 і 5 hours with the help of a mechanical oncometer according to A.S. Zakharevsky.

The experimental results indicated that the medicine under research exhibits a significant anti-edema action on the level of the reference medicine "Diclofen-gel". It has been experimentally found that the average anti-edema action of the "ArtpromentÒ" gel within 5 hours is 39 %, "Diclofen-gel" – 48 %; it is the evidence of their similarity in the absence of confidence in the index divergence. Therefore, the developed medicine shows the expressed anti-edema action at the level of 40-50 %, this property together with the analgesic and local anesthetic activities allows to recommend it for further study with the purpose of creation of the local medicine for traumatic injuries of the locomotor system treating accompanied with sports traumas (bruises, dislocations, sprains, ruptures of ligaments, tendons, etc.).

 

References

Derzhavnyi reiestr likarskykh zasobiv Ukrainy. Retrieved from http://www.drlz.kiev.ua [in Ukrainian].

Stefanov, O. V. (Ed.) (2001) Doklinichni doslidzhennya likarskykh zasobiv [Preclinical studies of drugs]. Kyiv: Avitsena. [in Ukrainian].

Drogovoz, S. M., Zupanets, I. A., Mokhort, M.A., Yakovleva, L. V., & Kliebanov, B. M. (2001) Eksperymentalne (doklinichne) vyvchennia farmakologichnykh rechovyn, yaki proponuiutcia yak nesteroidni protyzapalni zasoby [Experimental (preclinical) study of pharmacological agents that are offered as non-steroidal anti-inflammatory drugs]. Doklinichni doslidzhennia likarskykh zasobiv (O. V. Stefanov, Ed.), (p. 292–306). Kyiv: Avitsena. [in Ukrainian].

Zakharevskij, A. S. (1962). Vliyaniye nekotorykh proizvodnykh indola na nervuyu sistemu. (Dis … kand. med. nauk). [Influence of some indole derivatives nervous system]. (Candidate’s thesis). Minsk. [in Belarus].

Kovalenko, V. N. (Ed.). Kompendium 2013. Lekarstvennye preparaty [Compendium 2013 – drugs]. Kyiv: MORIOM. [in Ukrainian].

Lapach, S. N., Chubenko, A. V., & Babich, P. N. (2001). Statisticheskie metody v mediko-biologicheskikh issledovaniyakh s ispol`zovaniem Excel. [Statistical methods in biomedical research using Excel]. Kyiv: MORION. [in Ukrainian].

Maksimov, M. L. (2014). Mesto miorelaksantov v lechenii bolevogo sindroma [Fitting muscle relaxants in the treatment of pain]. Russkij medicinskij zhurnal, 7, 514–517. [in Russian].

Khabriev, R. U. (Ed.) (2005) Osnovnye metody statisticheskoj obrabotki rezul`tatov farmakologicheskikh eksperimentov [Basic statistical treatment of results of pharmacological experiments].Moscow: Meditsina. [in Russian].

Shpychak, O. S., Tykhonov, O. I., & Baranova, I. I. (2013) Patent 80917 Ukraina, MPK (2006.01) A61K 9/06, A61K 35/00. Ghel dlia mistsevogo likuvannia zapalnykh zakhvoriuvan oporno-rukhovogo apparatu na osnovi produktiv bdzhilnytstva. № u 2013 00514; zaiavl. 15.01.2013; opubl. 10.06.2013, Byul. 11.

Pykhlak, A. E`., Garkavi, A. V., Chursinova, I. I., Logachev, V. A., Mutieva, N. A., Kazbekova, O. N., & Nosovskyj, A. M. (2014) Primenenyie nimesulida v kompleksnom lechenii osteoartroza kolennykh sustavov [Application of nimesulide in treatment of osteoarthritis of the knee] Russkij medicinskij zhurnal, 7, 509–513. [in Russian].

Radko, I. M., Radko, A. I., Olevska, N. B., & Trofimchuk, M. Kh. (2005). Porivnialne vyvchennia zneboliuvalnoi dii mistsevykh anestetykiv [Comparative study of analgesic action of local anesthetics]. Medytsyna transportu Ukrainy, 3, 79–83. [in Ukrainian].

Rebrova, O. Yu. (2006) Statisticheskij analiz medicinskikh dannykh. Primenenie paketa prikladnykh programm STATISTICA [Statistical analysis of medical data. Application software package STATISTICA]. Moscow: MediaSfera. [in Russian].

Khabriiev, R. U. (2005). Rukovodstvo po e`ksperimental`nomu (doklinicheskomu) izucheniyu novykh farmakologicheskikh veshchestv [Guidelines for experimental (preclinical) study of new pharmacological substances]. Moscow: Meditsina. [in Russian].

Shpychak, О. S., & Tikhonov, O. I. (2014). Marketingovye issledovaniya farmacevticheskogo rynka sogrevayushchykh preparatov dlya mestnogo primeneniya pri zabolevaniyakh oporno-dvigatelnogo apparata [Marketing research pharmaceutical market warming preparations for topical use in diseases of the musculoskeletal system]. Ukrainskyi medychnyi almanakh, 17(2), 67–70. [in Ukrainian].

Shpychak, O. S., & Tykhonov, O. I. (2014). Teoretychne ta eksperymentalne obgruntuvannia skladu heliu protyzapalnoi ta mistsevoanestezuiuchoi dii dlia zastosuvannia v sportyvnii medytsyni [Theoretical and experimental study of anti-inflammatory gel composition and action of local anesthetic for use in sports medicine]. Farmatsevtychnyi zhurnal, 2, 54–63. [in Ukrainian].

Hanson, D., Allegrante, J. P., Sleet, D. A., & Finch, C. F. (2014). Research alone is not sufficient to prevent sports injury. Br. J. Sports. Med., 48(8), 682–684. doi: 10.1136/bjsports-2012-091434.

Mackey, A. L. (2007). Use of anti-inflammatory medication in healthy athletes – no pain, no gain? Scand. J. Med. Sci. Sports, 17(6), 613–614. doi 10.1111/j.1600-0838.2007.00738.x

Leppänen, M., Aaltonen, S., Parkkari, J., Heinonen, A., & Kujala, U.M. (2014). Interventions to Prevent Sports Related Injuries: A Systematic Review and Meta-Analysis of Randomised Controlled Trials. Sports Medicine, 44(4), 473–486. doi: 10.1007/s40279-013-0136-8.

Shpychak, О. S., & Tikhonov, O. I. (2014). Marketing research of pharmaceutical market of medicines used topically for diseases of locomotor apparatus. News of Pharmacy, 1(77), 63–68.

Van der Sluis, A., Elferink-Gemser, M. T., Coelho-e-Silva, M. J., Nijboer, J. A., Brink, M. S., Visscher, C. (2014). Sport Injuries Aligned to Peak Height Velocity in Talented Pubertal Soccer Players. Int. J. Sports. Med., 35(4), 351–355.

How to Cite

1.
Shpychak ОS, Tikhonov OI. The experimental study of the specific pharmacological action of «Artproment» combined gel. Zaporozhye medical journal [Internet]. 2014Sep.5 [cited 2024Mar.28];(4). Available from: http://zmj.zsmu.edu.ua/article/view/27460

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