Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma

Authors

  • Yu. A. Bisyuk
  • A. I. Kurchenko
  • V. E. Kondratiuk
  • A. I. Dubovyi

DOI:

https://doi.org/10.14739/2310-1210.2015.3.44506

Keywords:

Bronchial Asthma, Endotoxins, Polymorphism, Genetic

Abstract

Aim. The gene polymorphism of Asp299Gly TLR-4 may be associated with the risk of asthma development.

Methods and results. The gene polymorphism of TLR-4 (Asp299Gly) receptor has been researched in 262 early-onset and in 69 late-onset asthma patients. The state of anti-endotoxin immunity was assessed by determination of specific antibodies to the endotoxin of A, M, G classes and sCD14 by ELISA. The polymorphism was analyzed by the allele-specific polymerase chain reaction with electrophoretic detection. It was estimated that the risk of early-onset asthma in the population of Crimea is associated with genotypes AG and GG (Asp299Gly) of TLR-4. There were increased levels of anti-endotoxin IgM and decreased of sIgA in patients with late-onset asthma and AA genotype as compared to other genotypes.

Conclusion. The gene polymorphism of Asp299Gly TLR-4 is associated with the risk of early-onset asthma development in Crimea population.

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How to Cite

1.
Bisyuk YA, Kurchenko AI, Kondratiuk VE, Dubovyi AI. Immunity to endotoxin and Asp299Gly polymorphism of TLR-4 in adult patients with early and late onset of asthma. Zaporozhye Medical Journal [Internet]. 2015Jun.15 [cited 2024Jul.20];17(3). Available from: http://zmj.zsmu.edu.ua/article/view/44506

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Section

Original research