Estimation of the glutathione system parameters depending on the variant of the disease course in patients with newly diagnosed pulmonary tuberculosis at coinfection tuberculosis/HIV

Authors

  • R. M. Yasinskiy Zaporizhzhia State Medical University,

DOI:

https://doi.org/10.14739/2310-1210.2016.2.69214

Keywords:

Tuberculosis, HIV, Coinfection, Disease Progression, Glutathione, Glutathione Peroxidase, Glutathione Reductase, Glutathione Transferase

Abstract

The aim was to estimate the thiol-disulfide compounds depending on the variant of disease course in tuberculosis/HIV co-infected patients with newly diagnosed tuberculosis (NDT/HIV). Materials and methods. The study involved 54 patients with NDT/HIV, who were treated in the clinic of Zaporizhzhia regional clinical tuberculosis dispensary during 2010 – 2014 (average age 37,8 ± 1,2 y), 41 (75,9%) men, 13 (24,1%) women. Focal tuberculosis was diagnosed in 5 cases (9,3%), infiltrative – in 25 (46,3%) cases, disseminated – in 24 (44,4%) cases. The control group included 32 healthy individuals – blood donors (average age 35,9 ± 2,5 y), 22 (68,7%) men, 10 women (31,3%). Patients depending on the results of the treatment were retrospectively were divided into 3 groups: 1 – 15 patients with positive dynamics, 2 - with progressive disease course without systemic inflammatory response syndrome (n=13), 3 –with progressive disease course with SIRS (n=26).

Results. Levels of aldehyde-phenylhydrazone in 2nd and 3rd groups were significantly higher than in control in 1,3 and 1,2 times accordingly (p˂0,01 for both values), in the 3rd group they were higher in 1,1 times than in group 1, p˂0,05. Ketone-phenylhydrazone levels were higher in all groups compared with the control, in the 3rd group patients they were 1,1 times higher than in group 1, p˂0,05. The malondialdehyde level in 3rd group patients was higher than other groups parameters in 1,4 times in comparison with 1-t (p˂0,005) and 1.2 times – with 2nd group (p˂0,05).

Conclusion. Imbalance in the “oxidants-antioxidants” system both through increased free radical peroxidation, and because of thiol-disulfide balance shifts at the disease progression was detected.

References

Zhadan, V. M. (2012). Vyvchennia funktsionalnoho stanu hlutationzalezhnykh fermentiv erytrotsytiv u khvorykh na idiopatychni interstytsialni pnevmonii [The study of the functional state of erythrocytes glutathione-dependent enzymes in patients with idiopathic interstitial pneumonia]. Tuberkuloz, lehenevi khvoroby, VIL-infektsiia, 3(10), 36–41. [in Ukrainian].

Kulinskij, V. I., Kolesnichenko, L. S., Shprakh, V. V., Verlan, N. V., Bardymov, V. V., Gubina, et al. (2005). Izuchenie glutationa i fermentov ego metabolizma u bol'nykh starshikh vozrastnykh grupp s khronicheskoj cerebral'noj ishemii [Study of glutathione and its metabolic enzymes in patients of older age groups with chronic cerebral ischemia]. Byulleten' Vostochno-Sibirskogo nauchnogo centra SO RAMN, 1(39), 63–65. [in Russian].

Tolpygina, O. A. (2012). Rol' glutationa v sisteme antioksidantnoj zashchity (obzor) [Role of glutathione in the antioxidant defense system (review)]. Byulleten' Vostochno-Sibirskogo nauchnogo centra SO RAMN, 2–2(84), 178–180. [in Russian].

Gouripur, T., Desai, P. B., Van, A., Gouripur, K., & Patil, V. (2012). Comparison of lipid peroxidation product and enzymatic antioxidants in newly diagnosed pulmonary tuberculosis patients with and without human deficiency virus infection. International journal of pharma and bio sciences, 3(3), 391–397.

Connell, N. D., & Venketaraman, V. (2009). Control of mycobacterium tuberculosis infection by glutathione. Recent patents on anti-infective drug discovery, 4(3), 214–226. doi: 10.2174/157489109789318541.

Ghezzi, P. (2011). Role of glutathione in immunity and inflammation in the lung. International journal of general medicine, 4, 105–113. doi: 10.2147/IJGM.S15618.

Awodele, O., Olayemi, S. O., Nwite, J. A., & Adeyemo, T.A. (2012). Investigation of the levels of oxidative stress parameters in HIV and HIV-TB co-infected patients. The journal of infection in developing countries, 6(1), 79–85.

Venketaraman, V., Rodgers, T., Linares, R. Reilly, N., Swaminathan, S., Hom, D., et al. (2006). Glutathione and growth inhibition of mycobacterium tuberculosis in healthy and HIV infected subjects. AIDS research and therapy, 3(2). doi: 10.1186/1742-6405-3-5.

Downloads

How to Cite

1.
Yasinskiy RM. Estimation of the glutathione system parameters depending on the variant of the disease course in patients with newly diagnosed pulmonary tuberculosis at coinfection tuberculosis/HIV. Zaporozhye Medical Journal [Internet]. 2016May23 [cited 2024Nov.23];18(2). Available from: http://zmj.zsmu.edu.ua/article/view/69214

Issue

No. 2 (2016): Zaporozhye medical journal

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.  Лицензия Creative Commons
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access)