Fatty acid composition of total lipids and phospholipids of muscular tissue and brain of rats under the impact of vibration


  • N. M. Kostyshyn Lviv National Medical University,
  • M. R. Grzegotsky Lviv National Medical University,
  • J. F. Rivis Institute of Agriculture in the Carpathian region NAAS of Ukraine,




Fatty Acids, Phospholipids, Vibration, Muscle, Brain


Fatty acids are important structural components of biological membranes, energy substrate of cells involved in fixing phospholipid bilayer proteins, and acting as regulators and modulators of enzymatic activity. Under the impact of vibration oscillations there can occur shifts in the ratio of different groups of fatty acids, and degrees of their saturation may change. The imbalance between saturated, monounsaturated and polyunsaturated fatty acids, which occurs later in the cell wall, disrupts fluidity and viscosity of lipid phase and causes abnormal cellular metabolism.

Aim. In order to study the impact of vibration on the level of fatty acids of total lipids in muscular tissue and fatty acid composition of phospholipids in muscles and brain, experimental animals have been exposed to vertical vibration oscillations with different frequency for 28 days.

Methods and results. Tissues fragments of hip quadriceps and brain of rats were used for obtaining methyl esters of fatty acids studied by the method of gas-liquid chromatography. It was found that the lipid content, ratio of its separate factions and fatty acid composition in muscular tissue and brain of animals with the action of vibration considerably varies. With the increase of vibration acceleration tendency to increase in absolute quantity of total lipids fatty acids can be observed at the account of increased level of saturated and monounsaturated ones. These processes are caused by activation of self-defense mechanisms of the body under the conditions of deviations from stabilized physiological norm, since adaptation requires certain structural and energy costs. Increase in the relative quantity of saturated and monounsaturated fatty acids in phospholipids of muscles and brain and simultaneous reduction in concentration of polyunsaturated fatty acids are observed.

Conclusion. These changes indicate worsening of structural and functional organization of muscles and brain cell membranes of rats due to increased viscosity of lipids and changes in their intramembrane dynamics.


Hzhehotskyi, M. R., Fedorenko, V. I., & Shtabskyi, B. M. (2008). Narysy profilaktychnoi medytsyny [Essays of prophylactic medicine]. Lviv, Medytsyna i pravo [in Ukrainian].

Hopanenko, O. O., Rivis, Yu. F. (2013). Zhyrnokyslotnyi sklad fosfolipidiv plazmy krovi i tkanyn za hostroho arhininovoho pankreatytu ta yoho korektsii [Fatty acid composition of phospholipids of the blood plasma and tissues of rabbits with acute arginine pancreatitis and it`s correction]. Eksperymentalna ta klinichna fiziolohiia ta biokhimiia, 2, 22‒27. [in Ukrainian]

Gubskyi, Yu. I., Yanitska, L. V., & Briuzhina, T. S. (2005). Zhyrnokyslotnyi sklad lipidiv holovnoho mozku shchuriv pry toksychnomu urazhenni 1,2-dykhloretanom ta vvedennya nikotynamidu [Fatty acid composition of lipids in the rat brain lesions toxic 1,2-dichloroethane and the introduction of nicotinamide]. Suchasni problemy toksykolohii, 1, 19‒22. [in Ukrainian]

Cherniuk, V. I., Nazarenko, V. I., Karnaukh, M. H., et al. (2000) Derzhavni sanitarni normy vyrobnychoi zahalnoi ta lokalnoi vibratsii: DSN3.3.6.038-99 [Public health standards of industrial general and local vibration: PHS]. Kyiv [in Ukrainian]

Dotsenko, O. I., & Mischenko, A. M. (2011). Vplyv nyzkochastotnoi vibratsii na kyslotnu rezystentnist erytrotsytiv [Influence of low-frequency vibration on the erythrocytes acid resistance]. Visnyk Dnipropetrovskoho universytetu, 19(1), 22–30. [in Ukrainian]

Dlyaboha, Yu. Z., & Rivis, Y. F. (2011). Zhyrnokyslotnyi sklad fosfolipidiv plazmy krovi, pechinky i skeletnykh miaziv shchuriv za eksperymentalnoi hiperkholesterynemii ta vplyvu rybiachoho zhyru [Fatty acids composition of blood plasma, liver and skeletal muscules of rats with experimental hypercholesterinemia and under influence of fish oil]. Biolohichni studiyi, 2, 73‒84. [in Ukrainian]

Gula, N. M., & Margitich, V. M. (2009). Zhyrni kysloty ta yikh pokhidni pry patolohichnykh stanakh [Fatty acids and their derivatives in pathologic states]. Kyiv: Naukova dumka. [in Ukrainian]

Rivis, Y. F., & Fedoruk R.S. (2010). Kilkisni khromatohrafichni metody vyznachennya okremykh lipidiv i zhyrnykh kyslot u biolohichnomu materiali [Quantitative chromatographic methods for determining individual lipids and fatty acids in biological material]. Lviv. [in Ukrainian]

Rivis, Y. F., & Yanovych, N. E. (2014). Obmin zhyrnykh kyslot u pechintsi ta rist koropiv za riznoho rivnia tsynku ta midi u kombikormi [Fatty acids metabolism in liver and carps growth at different zinc and copper level in combined feed]. Naukovyi visnyk LNUVMBH im. Gzhytskoho, 3(60), 264‒273. [in Ukrainian]

Shtabskyi, B. M., & Hzhehotskyi, M. R. (1991) Ksenobiotyky, homeostaz i khimichna bezpeka liudyny [Xenobiotics, chemical homeostasis and human security]. Lviv: Nautilus [in Ukrainian]

Calder, P. (2012). Mechanisms of action of (n-3) fatty acids. J. Nutr., 142, 592‒599. doi: 10.3945/ jn.111.155259

Calder, P. C. (2013). Omega-3 polyunsaturated fatty acids and inflammatory processes: nutrition or pharmacology? Br. J. Clin. Pharmacol. 75, 645‒62. doi: 10.1111/j. 1365- 2125.2012.04374.x

Draeger, A., Monastyrskaya, K., & Babiychuk, E. B. (2011). Plasma membrane repair and cellular damage control: the annex in survival kit. Biochem. Pharmacol. 81(6), 703–712. doi: 10.1016/j.bcp.2010.12.027

Siasos, G., Tousoulis, D., Oikonomou, E., Zaromitidou, M., Verveniotis, A., Plastiras, et al. (2013). Effects of omega-3 fatty acids on endothelial function, arterial wall properties, inflammatory and fibrinolytic status in smokers: a cross over study. Int. J. Cardiol., 166, 340–346. doi: /10.1016/j.ijcard.2011.10.081

Schwenk, R. W., Holloway, G. P., Luiken, J. J., Bonen , A., & Glatz, J. F. (2010). Fatty acid transport across the cell membrane: regulation by fatty acid transporters. Prostaglandins Leukot. Essent. Fatty Acids, 82(4‒6), 149‒154. doi: 10.1016/j.plefa.2010.02.029

Levitan, I. B. & Kaczmareck, L. K. (1991). The Neuron, Cell and Molecular Biology. New York: Oxford Univ. Press.

Mallampalli, R. K., Ryan, A. J., Carroll, J. L., Osborne, T. F., & Thomas, C. P. (2002). Lipid deprivation increases surfactant phosphatidylcholine synthesis via a sterol-sensitive regulatory element within the CTP: phosphocholine cytidylyltransferase promoter. Biochem. J., 362, 81‒88. doi: 10.1042/bj3620081

Levy, B. D., Clish, C. B., Schmidt, B., Gronert, K., & Serhan, C. N. (2001). Lipid mediator class switching during acute inflammation: signals in resolution. Nature Immunol., 2, 612–619. doi: 10.1038/89759

Jump, D. B., & Clarke, S. D. (1999). Regulation of gene expression by dietary fat. Annu. Rev. Nutr., 19, 63‒90. doi: 10.1146/annurev.nutr.19.1.63

MacDonald, J. I., & Sprecher, H. (1991). Phospholipid fatty acid remodeling in mammalian cells. Biochim. Biophys. Acta., 1084, 105‒121. doi: 10.1016/0005-2760(91)90209-Z

(2001). Mechanical Vibration - Measurement and evaluation of human exposure to hand transmitted vibration, Part 1: General Requirements Medicine. Mechanical Vibration and Shock. London (British standart). International Organization for Standardization (ISO) 5349-1.

(1997). Mechanical Vibration and Shock-Evaluation of Human Exposure to Whole-Body Vibration ‒ Part1: General Requirements. Geneva, Switzerland. International Organization for Standardization (ISO) 2631–1: 1985 (E).

(1997). Mechanical Vibration and Shock-Evaluation of Human Exposure to Whole-Body Vibration ‒ Part2: Continuous and shock induced vibration in buildings (1 to 80 Hz). Geneva, Switzerland. International Organization for Standardization (ISO) 2631–2: 1985 (E).

Nakamura, M. T., & Nara, T. Y. (2004). Structure, function, and dietary regulation of delta 6, delta 5, and delta 9 desaturases. Annu. Rev. Nutr., 24, 345‒376. doi: 10.1146/annurev.nutr.24.121803.063211

Innis, S. M. (2007). Dietary (n-3) fatty acids and brain development. J. Nutr., 137, 855‒859.

Stein, W. D. (1986). Transport and diffusion across cell membrane. Orlando, MA: Academic Press.

Choi, Y. K., Cho, H., Seo, Y. K., Yoon, H. H., & Park, J. K. (2012). Stimulation of sub-sonic vibration promotes the differentiation of adipose tissue-derived mesenchymal stem cells into neural cells. Life Sci., 91, 329‒337. doi: 10.1016/j.lfs.2012.07.022

Wehland, M. A., Ma, X. M., Braun, M. C., Hauslage, J. D., Hemmersbach, R, Bauer, J, et al. (2013). The impact of altered gravity and vibration on endothelial cells during a parabolic flight. Cell Physiol. Biochem., 31, 432‒451. doi: 10.1159/000343380

Pre, D., Ceccarelli, G., Gastaldi, G., Asti, A., Saino, E., Visai, L., et al. (2011). The differentiation of human adipose-derived stem cells (hASCs) into osteoblasts is promoted by low amplitude, high frequency vibration treatment. Bone, 49, 295‒303.

doi: 10.1016/j.bone.2011.04.013

How to Cite

Kostyshyn NM, Grzegotsky MR, Rivis JF. Fatty acid composition of total lipids and phospholipids of muscular tissue and brain of rats under the impact of vibration. Zaporozhye Medical Journal [Internet]. 2016Sep.6 [cited 2024Jun.22];18(3). Available from: http://zmj.zsmu.edu.ua/article/view/77007



Problems of pharmacy