Indicators of cytological composition of the nasal mucus in children with acute respiratory viral infection

Authors

  • O. I. Smiyan Sumy State Medical University, Medical Institute, Ukraine,
  • Ye. V. Dmitrova Sumy State Medical University, Medical Institute, Ukraine,
  • V. A. Smiyanov Sumy State Medical University, Medical Institute, Ukraine,
  • O. P. Moshchich Shupyk National Medical Academy of Postgraduate Education,
  • I. Yu. Vysotskyi Sumy State Medical University, Medical Institute, Ukraine,

DOI:

https://doi.org/10.14739/2310-1210.2016.6.85521

Keywords:

Respiratory Infections, Adenoids, Nasal Mucosa, Children, Cellular Structures

Abstract

Aim. To study the cellular composition of the nasal secretion in children with acute respiratory viral infections in the acute phase of the disease.


Materials and methods. The study included 90 children aged 3 to 7 years with acute respiratory viral infections. Patients were divided into three groups. In the first group consisted of 25 healthy children (control group), the second group consisted of 33 patients with a diagnosis of acute respiratory viral infection, the third group – 32 patients with acute respiratory viral infection on the background of chronic adenoiditis. The cell populations of nasal secretion were studied, as well as the estimation of neutrophils and epithelial cells nuclei and cytoplasm destruction. Evaluation of destructive changes in the cellular composition of nasal secretion in children with acute respiratory viral infections was studied by the Matveeva’s method. (1993).

Results. Minimal nuclei and cytoplasm of epithelial cells and neutrophils damage were typical for patients of the control group. In children with acute respiratory viral infection partial destructive changes in the epithelial cells (p<0.001) and neutrophils (p<0.001) were observed. A deeper level of cellular destruction of neutrophils (IDC 0.72±0.005, p<0.001) and epithelial cells (IDC 0.67±0.007, p<0.001) were revealed at children with acute respiratory viral infection on background of chronic adenoiditis.


Conclusions. Increasing the index of epithelial cells and neutrophils degradation in the II children group indicates the activation of local immunity mechanisms. However, the full destructive changes in the cytoplasm and nucleus of neutrophils and epithelial cells indicate the performance of their effector functions in the focus of bacterial inflammation that is typical for children with acute respiratory viral infections with chronic adenoiditis. Cytological examination of nasal secretion due to minimal invasiveness and availability can be used for study of local immunity cellular component in children with acute respiratory viral infection and for disease diagnostics and control of their treatment.

References

Aref´eva, N. A., & Aznabaeva, L. F. (2009). Immunnye reakcii slizistoj obolochki nosa: citologicheskaya diagnostika, metody lecheniya [Immune reactions nasal mucosa: cytological diagnosis, treatment]. Consilium medicum, 11(11), 30–34. [in Russian].

Dmitrova, Ye. V. (2015) Osoblyvosti klinichnoho perebihu hostroi respiratornoi virusnoi infektsii u ditei u poiednanni z adenoidnymy vehetatsiiamy [Features of clinical course of acute respiratory infections in children in combination with adenoid vegetation]. Infektsiini khvoroby v practytsi likaria-internista: suchasni aspect. Proceedings of the All-Ukrainian Scientific and Practical Conference. (pp. 25–27). Sumy [in Ukrainian].

Majdannik, V. G., & Mitin, Yu. V. (2006). Diagnostika, lechenie i profilaktika vospalitel`nykh zabolevanij organov dykhaniya u detej [Diagnosis, treatment and prevention inflammatory diseases respiratory organs in children]. Kyiv. [in Ukrainian].

Chernyshova, L. I. Syniachenko, V. V., & Naumenko, N. V. (2001). Mukozalnyi imunitet ta yoho korektsiia pry infektsiiakh verkhnikh dykhalnykh shliakhiv [Mucosal immunity and its correction infections of the upper respiratory tract]. Perynatolohiia ta pediatriia, 3, 76–79. [in Ukrainian].

Mizernickij, U. L., & Ermakova, I. N. (2007). Sovremennye mukoaktivnye preparaty v terapii ostrykh respiratornykh zabolevanij u detej [Modern mucoactive drugs in the treatment of acute respiratory infections in children]. Pediatriya. Prilozhenie k zhurnalu Consilium-medicum, 1, 53–56. [in Russian].

Yulish, E. I. (2010). Faktory mestnogo immuniteta pri respiratornykh infekciyakh i metody ikh aktivacii [Factors of Local Immunity in Respiratory Infections and Methods of Their Activating]. Zdorov`e rebenka, 5(26), 63–67. [in Ukrainian].

Smiian, O. I., Dmitrova, Ye. V., & Vasylieva, O. H. (2015). Klinichni osoblyvosti perebihu ghostrykh respiratornykh virusnykh infektsii u ditei u poiednanni z patolohiieiu hlotkovoho myhdalyka [The clinical features of an acute respiratory viral infections in conjunction with pathology of pharyngeal tonsil in children]. Scientific Journal «Science Rise», 9/3(14), 68–74. [in Ukrainian]. DOI: 10.15587/2313-8416.2015. 50551.

Matveeva, L. A. (1993). Mestnaya zashchita respiratornogo trakta u detej [The local protection of the respiratory tract in children]. Тоmsк: Izdatel´stvo Тоmsкоhо universitetа. [in Russian].

Pikuza, O. I., Chalimova, L. F., Samorodnova, E. A., & Agafonova, E. V. (2010). Osobennosti mestnogo immuniteta pri ostrykh bronkhitakh i pnevmoniyakh u detei i metody ikh korrekcii [Peculiarities of local immunity at chidren with acute bronchitis and pneumonia and methods of their correction]. Prakticheskaya medicina, 5, 139–142. [in Russian].

Каrpov, А. А., Оreshko, L. S., Pashinin, А. N., Bezrukova, E. V., & Voronov, V. A. (2012). Sostoyanie slizistoj obolochki polosti nosa u bol`nykh stradayushchikh celiakiej [Condition of nasal mucosa in patients with celiac disease]. Zdorov`e – osnova chelovecheskogo potenciala: problemy i puti ikh resheniya, 1(7), 481–482. [in Russian].

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How to Cite

1.
Smiyan OI, Dmitrova YV, Smiyanov VA, Moshchich OP, Vysotskyi IY. Indicators of cytological composition of the nasal mucus in children with acute respiratory viral infection. Zaporozhye Medical Journal [Internet]. 2016Dec.8 [cited 2024Jul.23];18(6). Available from: http://zmj.zsmu.edu.ua/article/view/85521

Issue

No. 6 (2016): Zaporozhye medical journal

Section

Original research

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